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KMID : 0881720220370010021
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Journal of Food Hygiene and Safety
2022 Volume.37 No. 1 p.21 ~ p.28
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Optimal Conditions for the Production of Gamma-aminobutyric Acid by Enterococcus casseliflavus PL05 Isolated from Oenanthe javanica
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Choi Se-Mi
Kim Jeong-A
Kim Geun-Su
Kwon Do-Young
Kim Sang-Gu
Lee Sang-Yun
Lee Kang-Wook
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Abstract
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In this study, a new lactic acid bacterium (LAB) that could produce gamma-aminobutyric acid(GABA) was isolated from Oenanthe javanica (water celery) and identified as an Enteroccoccus casseliflavus strain.
Until recently, there have been many studies on the gamma-aminobutyric acid producing lactic acid bacterium, as wellas on some lactic acid bacterium in Enteroococcs genus, but none on the species E. casseliflavus. Therefore, in thepurpose of finding the optimal conditions for GABA production of E. casseliflavus PL05, the effects of several conditionsincluding the type of mediums, growth temperatures, initial pH, growth time, L-mono sodium glutamate(MSG) concentration, and carbon source were tested. The study revealed that the PL05 strain grew better in the BrainHeart Infusion (BHI) medium than in the Man, Rogosa, and Sharpe (MRS) or Tryptic Soy Broth (TSB) medium. Also,similar results were obtained with GABA production conditions. As a result of analysis on the GABA productionyield by concentration of MSG, a GABA substrate, the highest production was found at 7% of MSG concentration.
However, since similar level of production was found at 5%, it is considered to be more efficient to use 5% MSGconcentration. The analysis on the growth and GABA production yield by carbon sources showed the highest resultswhen maltose was used. From the final test under the optimal conditions found, 140.06±0.71 mM of GABA was producedover 24 hours with the conversion rate of 78.95%. Lastly, from the sensitivity analysis on the 10 different antibiotics,including vancomycin, it was found that there were not confirmed cases of resistance.
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KEYWORD
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Gamma-aminobutyric acid, Lactic acid bacteria, Enterococcus casseliflavus, Oenanthe javanica
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